Improvement of broiler chickens immune system by in ovo injection of 25(OH) D3 and K3 vitamins

This experiment was conducted to determin the effect of 25(H)D3 and K3 vitamins on immune system of Ross 308 broiler chicken in a completely randomized design with a 3×4 factorial arrangement with four replicates and 16 observation per replicate. Treatments included vitamine D3 (0, 0.4, 0.6 and 0.8 μg) and vitamin K3 (0, 2 and 6 μg). On day 180 g incubation 0.5 ml of treatment solution was injucted in fertile eggs. Male chicken were reared in experiment cages for a period of 42 days. To evaluate humoral immune system reaction 0.5 ml SRBC (10%) was injected on chickens’ breast muscle at the 28th and 35th day, thereafter chicken blood samples were taken on the 35th and 42nd day. In order to evaluate the cellular immune system reaction, Phyto-hemagglutinin (PHA) was injected in the curtain chickens toe on day 24 of rearing period and skin response were studied on 1 and 5 days after injection. The results showed that with increasing levels of vitamin K3, antibody titers against Newcastle were increased (P