Treatment of Prostate Cancer Cells (DU145) with Gamma-Ray Radiation and Silver Nano Particles
Abstract:
Background
Prostate cancer is the second most common cancer in America which causing great harm and waste cost. Furthermore almost more prostate cancer treatments are ineffective. Purpose of this study was to evaluate the effects of therapy and the rate of increase of the absorbed dose of gamma radiation with silver nano particles in treatment of prostate cancer.
Materials And Methods
DU145 cell line originating from Human prostate cancer was purchased from Pasteur Institute. After thawing of defreezed samples, cells were incubated with DMEM medium and 15% FBS Confluency FBS serum was added. For preventing contamination amphotericin B in 25 μM/ml was added. Cells were incubated over a period of 3 to 5 days to reach a good Confluency Then cells divided into 4 groups . Tentatively assigned to the control group, the second experimental group treated with gamma irradiation at a doses of 2, 6 and 10 Gy, the third group treated with 53 μg/ml ã g/mlµ 53 silver nano particles. The fourth group includes simultaneous treatment with gamma doses and silver nano particle.Çä˜æÈå æ Cell groups studied by staining with trypan blue as well as by MTT assay (ELISA) reader.
Results
The results showed the use of gamma rays and silver nano particles caused a significant reduction in the number of cancer cells in the treated groups compared to the other treatment groups and a control group. Using of silver nano particles as a radio sensitizer and radiation therapy in prostate cancer cell lines DU145 resulted in the increase of the gamma-ray photon energies of 6 and 10 Gy.
Discussion
The gamma photon radiation to the tumor cells were incubated nano particle probability Photoelectric process as a process when handling the extremely high energy gamma photons hitting the silver nano particles to cancer cells, photoelectron and Auger electrons (Auger Electrons ) and secondary electrons produced by secondary particles can be. It will not be very effective, leading to failures in the field of DNA damage and cell death.
Language:
Persian
Published:
Alborz University Medical Journal, Volume:5 Issue: 4, 2016
Pages:
209 - 217
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