The determination of different effective concentration of ethanolic extract of bee pollen on biochemical analysis in liver, spleen and heart tissues of rainbow trout, Oncorhynchus mykiss (Walbaum, 1792)

The aim of this study was to investigate the effective concentration of ethanolic extract of bee pollen on liver, spleen and heart tissues of fish. Bee pollen extract in various concentrations (0.5, 2.5, 5, 10, 20 and 30 ppm) was administered to fish for 96 h under aquarium conditions. The malondialdehyde (MDA) levels, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) and levels of total free sulfhydryl groups were investigated in liver, spleen and heart in fish samples. MDA levels in liver, spleen and heart tissues of various concentration groups (2.5, 5, 10, 20 and 30 ppm) decreased (p