Effect of Ethanol and Hexane Extract of Tribulus terrestris on Quality of Sperm and Histology of Males Rats

Inroduction & Infertility is a serious problem. About 10% of males are estimated facing the problem of fertility. Rapid growth and development of sciences within the pharmaceutical span and focus and rely on production of herbal medicines and the production of plant-based infertility drugs is growing . Today, a lot of research regarding infertility and the effects of herbs on it and finding the best method for plant extraction takes place. Plant is the main source in discovery of novel medicine. Nearly 40% of modern medicines are originated from plants.Tribulusterrestris is also an important herb that used for infertility. Water extract of T.terrestris contains steroidal saponins and protodioscin that increased sexualhormones and testosterone. Water extract of T.terrestris increased sexual desire and weight of prostate.
Material and In the present study, 80% hexane and ethanol extracts of Tribulusterrestris prepared and force feed to two groups of mice. Each group was consist of 4subgroups with 8 mice and extract with doses of 5, 10 and 20 mg per body weight was applied on them. After 45 days of treatment the mice were anesthetized and killed.
The present study showed that in comparison between ethanol and hexane extract of fruit of T. terrestis, in dosages 5, 10, 20 mg per weight of body, hexane extract, increased sperm count, but didn’t show any significant effecton control group. Instead,ethanol extract of fruit of T. terrestis not only increased sexualhormones as LH and testosterone but also increased spermatogenesis in lumen of seminiferous and changed histological of testis.This extract didn’t changethe weight of testis and epididymis.
According to the results, ethanol extraction of aloe vera as an antioxidant compound is able to reduce the levels of cardiovascular damage in mice treated with titanium dioxide nanoparticles.