Ghrelin localization in testicular and epididymis tissues of Ram
Abstract:
The aim of the present study was to immunohistochemical positioning of ghrelin in testicular and epididymis tissues of Kurdish ram. Ghrelin, a 28-amino acid acylated peptide is an important factor involved in most of the metabolic and hormonal signals which adapt the reproductive functions in conditions of altered energy balance. This study investigated the immunohistochemical localization of ghrelin in testicular and epididymis tissues of Kurdish ram, using mouse monoclonal anti ghrelin antibody as primary antibody and polyclonal donkey anti IgG Horseradish Peroxidase (HRP) antibody as secondary antibody. Samples of testis collected from three native male sheep aged of 1.5 to 2 years old, and preserved in 10% formalin for posterior inclusion in paraffin. Histological sections with 5 micron in thickness were prepared for IHC. Immunoreactions was assessed for Germ cells, Leydig and Sertoli cells and head of epididymis. It is believed that the site of ghrelin expression in the spermatogenesis process, Leydig, sertoli cells and head of epididymis may indicate its role in local (autocrine / paracrine) regulations. This is one of the first studies to provide molecular evidence for the presence of ghrelin within the entire testicular and epididymis tissues of ram.
Language:
Persian
Published:
Veterinary Researches and Biological Products, Volume:30 Issue:1, 2017
Pages:
75 - 81
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