A survay on Analytical and electrophretical pattern of Caspian Ponies Serum proteins

Caspian pony were lived in caspian sea shores from thousend years ago and they are entioned horses in word. Proteins are operated az enzyme, hormone, antibody and homostasis factores. .Addisionall blood samples were collected from 94 clinically healthy Caspian pony from Gilan, Mazandaran and golestan provience.and serum sample were dectede.Serum total protein were estimated by Elan biochemical analyzer made by Epedorf with Biuret method. Serum protein electrophoresis was performed using a Sebia K20 agarose gel electrophoresis system. For coparision whitin mean of potein in male and female ponies Coefficients of variation were calculated. Six fraction that observed were Albumin, α1glubulin, α2 glubulin,β1 glubulin,β2 glubulin and γ globulin. The mean of total protein were 7.34±0.8, Albumin 3.68±0.03, α1 glubolin 0.19±0.004, α2 glubolin0.46±0.01, β1 0.71±0.02, β2 glubolin0.78±0.01, total glubolin3.65±0.05. Coffman (1969) and Osbaldisston(1972) in survey on 20 and 62 horses reported α1,α2,β1,β2 glubolin. Massip and fommier(1974) in survey on 98 horses and Cerek et al from 14 horses reported β1,β2,α2c and α1ab and α1 and α2 reported.