In vitro susceptibility of pseudomonas aeurogenosa isolated from urinary tract of patients with uti to doxycycline and tigecyclin and comparison with other anti pseudomona antibiotics

Pseudomonas aeruginosa is a gram negative bacillus. Infection with these bacteria usually occurs in patients who hospitalized due to: need for prolonged stay in ICU, those who have intravascular catheters, or indwelling urinary catheters. This Study investigates in vitro susceptibility of pseudomonas aeruginosa which were isolated from patients with UTI to doxycycline, tigecycline, ceftazidime, cefepime, piperacillin/tazobactam, impipenem, meropenem, colistin, ciprofloxacin, nitrofurantoin, amikacin) with standard disk diffusion. Comparing the, susceptibility results of disk diffusion with E.test for doxycycline and tigecycline is another aim. Other purpose of this study was to define MDR pseudomonas aeruginosa susceptibility to doxycycline and tigecycline .

Sixty pseudomonas aeruginosa which was isolated after biochemical and bacteriological test, were entered to study and susceptibility to above antibiotics was tested by disk diffusion. For doxycycline and tigecycline antibiogram by two methods were performed: disk diffusion and Etest.

Maximum susceptibility was observed for colistin =95% followed by sensitivity in decreasing order for these antibiotics .Meropenem 76% Imipenem= Cefepime= Ceftazidime 63.3% Piperacillin/tazobactam 55% doxycycline 46% amikacin 45% Ciprofloxacine 35% Tigeycline 15% nitrofurantoin 0%. Measurement of kappa for agreement of two methods of antibiograms revealed moderate agreement in this regard.

In vitro susceptibility of isolated pseudomonas aeruginosa to studied antibiotics (with exception of colistin) was less than 80% even for carbapenems. Disk diffusion for doxycycline and tigecycline wasn’t effective for differentiation intermediate resistance from sensitive. Calculation of cohens Kappa for agreement was moderate. Most of MDR pseudomonas aeruginosa was resistant to doxycycline and tigecycline.