Effect of three probiotics (Lactobacillus plantarum, L. casei and Bacillus subtilis) on growth and survival of Artemia franciscana nauplii against pathogenic bacteria, Vibrio campbellii, in gnotobiotic condition

The effect of three probiotics (Lactobacillus plantarum, L. casei and Bacillus subtilis) on growth and survival of artemia against a pathogenic bacteria, Vibrio campbellii, in gnotobiotic conditions were investigated. A total of 1560 nauplii were equally transferred to 78 falcon tube containing 30 ml of autoclaved sea water with 70 ppt salinity. Treatments consisted of a control (alga, Dunaliella tertiolecta) and 12 treatments of 3 bacteria (each with 25%, 50%, 75%, and 100% replacement) and each treatment was done in 6 replicates. On the sixth day, survival rate, length and the number of bacterial colonies in the nauplius were studied. Also, in this day the nauplius was challenged with the pathogenic bacteria, V. campbellii. The maximum length of the nauplius was observed in the group supplied with 25% L. plantarum and 75% of alga (P