Biomass and growth of green algae Chlorococcum sp. using organic manures and soil extract

In order to determine effects of chicken and cattle manures in culture of Chlorococcum, an experiment was designed in six treatments including; 0.1, 0.4, 0.8 g/l of chicken manure and 0.1, 0.4, 0.8 g/l of cattle manure as completely randomize design with three replicates for 28 days. Results showed that the mean maximum density (87.1 × 105 cell/ml), specific growth rate (0.054 day-1), algal dry biomass (0.644 g/l), and chlorophyll a (9.42 mg/l) were obtained with 0.8 mg/l chicken manure. In order to compare performance of these manures with other culture media, second experiment with five treatments including; BBM (control) (Bold’s Basal Medium), BBM soil extract, 0.8 g/l chicken manure, 0.8 g/l cattle manure and mixture of all treatment (BBM, BBM soil extract, chicken manure and cattle manure) was designed as completely randomize design with three replicates for 15 days. Comparative results showed that BBM soil extract had highest algal density (11.6 x 106 cells/ml), highest algal dry biomass (0.81 mg/ml), maximum SGR (0.13 /day), highest chlorophyll a (10.15 mg/l) and minimum doubling time (4.97 days). In conclusion, performance of BBM soil extract was better in terms of biomass and growth parameters of Chlorococcum.