Evaluation of Four Obesity-Related Genes Polymorphism in Obese Women Residing in East Azerbaijan, Iran

In view of the fundamental role of genetics in development of obesity, the present study aimed to investigate the single nucleotide polymorphism of some obesity-related genes among a subset of obese women living in Tabriz, Iran. For this purpose, 70 eligible obese women (aged 18-45 years) were genotyped for the uncoupling protein-1 (UCP-1) -3826A>G, ß3-adrenergic receptor (ß3ADR) Trp64Arg, leptin G-2548A and adiponectin T>G polymorphisms. Accordingly, genomic DNA was isolated from whole blood samples using the conventional phenol chloroform extraction method and the single-nucleotide polymorphism of leptin, adiponectin (ADIPOQ), UCP-1 and ß3ADR genes were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The frequencies of GG, GA, and AA genotypes for leptin polymorphism G-2548A were found 20.0%, 51.4% and 28.6%, respectively. The genotype frequencies of TT, TG, and GG for adiponectin polymorphism T>G were found 67.1%, 27.1% and 5.7%, respectively. The genotype frequencies of AA, AG, and GG for UCP-1 polymorphism A-3826G were found 48.6%, 32.9% and 18.6%, respectively. Finally, the genotype frequencies of Trp64Trp, Trp64Arg, and Arg64Arg for ß3ADR gene were found 84.3%, 15.7%, and 0%, respectively. The genotype-allelic frequencies of ADIPOQ and ß3ADR genes were almost the same in different populations, while leptin and UCP-1 genes had different genotype distributions. Since the aforementioned genetic variations have important roles in development of obesity and also response to treatments like weight lowering diet, it is worth studying the genotype of every population before conducting any interventional program for the obesity management.