Infection Determine of Ornamental Plants to Some Important Viral Diseases in North Khorasan Province by ELISA

In recent years, the symptoms of viral diseases such as Mosaic, Paleness and Ring spots have spread on ornamental plants of North Khorasan province. In order to detect important viral diseases in ornamental plants of North Khorasan Province, a total of 400 samples of ornamental plants were collected and transported to the laboratory. All of the samples were evaluated by DAS-ELISA serological test with polyclonal antibodies to Tomato spotted wilt virus (TSWV), Tomato ring spot virus (ToRSV), Tomato bushy stunt virus (TBSV), Tomato mosaic virus (ToMV), Cucumber mosaic virus (CMV) and Alfalfa mosaic virus (AMV). The positive Samples in ELISA test were inoculated on indicator plants for observation of the symptoms. All the indicator plants distinctive symptoms of diseases in the mechanical inoculation. The results of the ELISA test showed that 211 samples of total 400 ornamental plant samples, were infected with different viruses. Also the results indicated that among 16 ornamental species including Tagetes signata, Tagetes patula, Gaillardia pulchella, Calendula officinalis, Chrysantheum indicum, Zinnia elegans, Leucanthemum superbum, Ipomoea tricolor, Ipomoea batatas, Rosa hybrid, Pelargonium hortum, Amaranthus caudatus, Petunia hybrid, Berberis thunbergii, Lavandula angustifolia, and Symphoricarpus racemosus; 9 species infected and 7 species were virus free. Of the six viruses of infecting ornamental plants, TBSV and ToRSV had the highest and lowest infection frequency (incidence) in this province respectively, and infection with AMV was not observed in any of the tested samples.