An Investigation Of Genetic Diversity Urtica dioica L. in Central area of Mazandaran provenience by RAPD marker

Aim and Urtica dioica L. is used as medicinal plants to cure some disease such as; digestives disease or declined blood sugar and hypertension. It is known that by the increasing of population the confirmed need of pharmacy industries to medicinal plants as well as, the importance of their effective materials in nutritional adornment and hygienic are caused them to deserve special attention and research at the point of cultivation, production and consumption. In this research sample plants were collected central area of Mazandaran province was studied in aspect of genetic variation.
In order to evaluate the genetic diversity, the genomic DNA was extracted by using modified CTAB protocol. The evaluation of quality and quantity of DNA was examined with agaros gel electrophoresis. Six RAPD markers have been used for PCR analysis.
Out of 155 RAPD bands, 68/38% were polymorphism. The data were analyzed with NTSYS programs. The dandrogram was drawn based on UPGMA.
Investigation the genetic variation of this plant with RAPD indicated that this marker is suitable to determine the polymorphic loci and to estimate the genetic distance.