The Impact of Lead Stress on Proline, Soluble Sugars, Antioxidant ‎Enzyme Activities, and Growth Parameters in Canola Hyolla401

As one of the most dangerous heavy metals in the world, Lead impacts the metabolic and physiological activities of plants. In this study, the effect of Lead stress on proline and antioxidant enzymes activity in the roots and shoots of Hyola401 was examined in hydroponic solution. A completely randomized design experiment with three replications per treatment was carried out. Statistical analyses were conducted using SPSS software and Duncan’s test of comparison of means. Three levels of Lead took place by Pb (NO3)2 (0, 100, & 200 micromoles per liter). After seven days of treatment, plants were collected for laboratory analysis. The results showed that an increase in the concentration of Lead increased proline, soluble sugars, catalase and peroxidase activity in roots and shoots; whereas it decreased the growth parameters. These increases are an indication of plant resistance against Lead stress and play a determining role in reducing the side effects of oxidative damages.