Inter-Simple Sequence Repeat loci Associations with Predicted Breeding Values of Body Weight in Kermani Sheep
Author(s):
Abstract:
The Kermani sheep is an important meat producing animal in Iran and its economic efficiency is mainly dependent on its growth and reproduction ability. Inter Simple Sequence Repeat (ISSR) is the genome region between microsatellite loci. The aim of this study was to evaluate the association of two different ISSR markers with predicted breeding values (PBV) of body weight trait in Kermani sheep. Blood samples were obtained from 240 Kermani sheep. Polymerase chain reaction (PCR) was performed using two ISSR primers (GA)9C and (AG)9C. The amplified PCR fragment sizes ranged from 100 to 3100 bp and primers amplified 28 (A1 to A28) and 36 (G1 to G36) fragments, respectively. In addition, breeding values for birth weight, weaning weight (at 3 months of age) and body weight at 9 months of age for all Kermani sheep were predicted by univariate analysis of the animal mixed model. Associations of the loci with predicted breeding values of body weights were evaluated using a general linear model. Five polymorphic ISSR loci, G14, G25, A8, A20 and A26 had significant associations with PBVs of body weight (P
Keywords:
Language:
English
Published:
Genetics in the Third Millennium, Volume:14 Issue: 4, Autumn 2016
Pages:
4383 to 4390
https://www.magiran.com/p1684636
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