The survey on presence of methicillin-resistant gene (mecA) in staphylococcus aureus isolates from food origin
Message:
Abstract:
Staphylococcus aureus has become a major concern because of the ability to acquire resistance to antimicrobial agents. The aim of this study was to survey methicillin-resistant gene (mecA) in Staphylococcus aureus isolates from food origin and determine their antimicrobial susceptibilities pattern. For this purpose, 146 food samples were evaluated. Also 34 isolates of Staphylococcus aureus with food origin were used available in the archives of the faculty. All isolates were cultured and evaluate by morphology and biochemical characteristics of Staphylococcus aureus. All isolates were subjected for two PCR. The first one was done to confirm Staphylococcus aureus species according to amplify the nuc gene specific for Staphylococcus aureus. The second PCR was done for evaluate the presence of methicillin resistant gene (mecA) in isolates. Antimicrobial susceptibility of staphylococcus aureus isolates was determined using the Kirby–Bauer disc diffusion method. The result of the first PCR confirmed that all of the isolates were positive for the nuc gene. Second PCR for the presence of mecA gene showed that out of 66 coagulase positive Staphylococcus aureus, only 1 isolate (1.51%) had the mecA gene. Antibiotic susceptibility results showed that the highest resistance was to penicillin (34.84 %) followed by resistance to Azitromycin and Erytromycin (10.6%), Oxacillin (7.57%), Trimethoprim and Sulfametoxazol (6.06%), Cloxacillin (4.54%) and Cyprofloxazin, Meropenem and Florfenicole(1.51%). No resistance was found to Rifampin, Ceftizoxime, chloramphenicol, nitrofurantoin, gentamicin and novobiocin. The isolate with mecA gene was resistant to Penicillin and Oxacillin and had relative sensitivity to Cloxacillin, Trimethoprim, Sulfametoxazol, Meropenem and Methicillin.
Language:
Persian
Published:
Food Science and Technology, Volume:14 Issue: 2, 2017
Pages:
303 to 313
magiran.com/p1684786  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!