Induced mutagenesis using sodium azide (NAN3) on sugar cane regeneration from callus in variety CP69-1062

In order to investigate the mutagenicity of mutagenic substances NAN3 CP69-1062 in sugarcane varieties in different amounts on the first round callus obtained from leaf meristem were applied. In the first six Sterilization treatment with four replications in a completely randomized design on the primary leaf explants derived from the meristem were applied. 5 treatments with 4 replications in a completely randomized design callus explants for callus induction from the primary leaves were studied. 2,4- D to a value of 4 and 3 mg resulted in callus were highest. For induction of mutation on differentiated cells obtained from primary leaves regression equation Y= 5.84.78 X presented. Using calculated equation, LD50 was calculated and as 0.34 mm was introduced as dose that resulted in %50 mortality of callus. Also, for callus regeneration, 6 treatments of regeneration were evaluated in factorial experiment based on completely randomized design with four replications. Kin hormone levels in the second and third levels of NAA in the above treatments formed. 3 milligrams per liter of Kin and 0.1 milligrams per liter of NAA were significant different from other treatments at α= 0.01. Rooting shoots in the basic MS medium with half the nitrate concentration of auxin IBA and NAA with different values of the two had been completed, was conducted. The results showed that the basic MS medium with half the concentration of nitrate was completed, along with NAA at a rate of 0.01 mg greatest impact on rooting germ have been regenerated.