Sub-lethal effects of nano zinc oxide (ZnO NPs) on some hematological indices of goldfish (Carassius auratus)

Extensive development of nanotechnology and applications of nano-particles in different industries has caused devastating effects of nano-materials on organisms. This study aimed to evaluate the effects of nano zinc oxide on hematological factors of goldfish.
In this paper we determine the range of concentrations of zinc oxide nano-particles to determine LC50 of gold fish at 24, 48, 72 and 96 hours respectively. Then, according to the LC50, separate experiments were designed to induce sub-lethal concentration of this substance on Hematological parameters were studied. Blood samples of goldfish fry were taken in the 7 days following exposure to lethal concentrations (50% concentration) of nano-ZnO nano-materials with fish that were not exposed.
Findings: The results showed that the nano-particles could changes in blood parameters of gold fish by increasing red blood cell (RBC), hemoglobin, hematocrit, MCV in treatment was influenced by the nano-particles, was (P>0.05) and reduced white blood cell (WBC) in the treatment of fish exposed to lethal concentrations of nano-particles compared to the control group (P>0.05) but did not show significant change in MCH, MCHC indices.
Discussion and In conclusion sub-lethal concentration of nano zinc oxide could affect hematological parameters of goldfish and this fish have more resistance to this kind of pollutant.