Evaluation the effects of different levels of vitamin E and Nano Selenium on sperm quality parameters of Leghorn rooster during chilled storage on 4◦C

Using antioxidants can diminish sperm damages during chilled storage.
The present study was designed to evaluate the effect of vitamin E and Nano-selenium on improvement parameters of rooster semen during chilled storage.
METHODES: Semen was collected from twenty White Leghorn roosters and diluted with extender containing with no antioxidants (control), or containing 5 (E1), 10 (E2) µg/mL vitamin E or containing 0.5% (NS1), 1% (NS2) and 2% (NS3) Nano-selenium and then chilled in 4◦C. Sperm viability, total motility, Progressive motility, plasma membrane integrity and abnormality were evaluated at 0, 24, 48 and 72 h after storage. Also, amount of malondialdehyde (MDA) was assessed in seminal plasma at the end of experiment.
Sperm viability (%), total and progressive sperm motility (%) as well as sperm plasma membrane integrity significantly decreased among the time (P With regard to these results, addition of 5 and 10 µg/mL vitamin E as well as 0.5 and 1% Nano-selenium can be improved sperm quality and decreased sperm oxidative damages during chilled storage.