Molecular survey of the frequency of sea and seb genes in ‎Staphylococcus aureus isolated from skin infections in Razi hospital ‎of Tehran

Staphylococcus aureus is one of the most common nosocomial and community ýacquired pathogens. The bacterium has different virulence factors and provides aggressive conditions ýto the host with secretion of the toxins such as super antigenic enterotoxins. Staphylococcal enterotoxin ýA and B (SEA,SEB) have the most severe toxic effect among these toxins. The aim of this study was ýto determine the frequency of A and B enterotoxin genes in Staphylococcus aureus isolated from skin ýinfections in Tehran Razi hospital. ý
A total of 65 isolates of Staphylococcus aureus from skin samples were ýcollected and confirmed with phenotypic methods and checked by Polymerase Chain Reaction (PCR) ýusing species specific primers. Then the frequency of the sea and seb genes were detected with PCR ýusing specific primers. ý
The PCR results showed that 86.20 % of Staphylococcus aureus isolates carried the sea ýgene and the frequency of seb gene in the tested isolates was 15.40 %. ý
According to the importance of Staphylococcus aureus enterotoxins and their role in the ýdevelopment and exacerbation of the staphylococcal diseases, the presence and expression of the ýcorresponding genes in clinical isolates must be considered in management of the diseases.ý Keywords: Staphylococcus aureus, Enterotoxins A and B, Skin infections.ý