Improvement of asymbiotic germination of Epipactis veratrifolia

For improvement asymbiotic seed germination media of temperate terrestrial orchid Epipactis veratrifolia , 12 different concentrations of the carbohydrates (fructose, glucose, sucrose and two combination of fructose with sucrose ) were assessed on the seed germination and protocorm development, in the presence (2g L−1) and absence of peptone in Fast medium. Results revealed significant differences between treatments on seed germination percentage and protocorm growth. Carbohydrate treatments; H12 (3.5g/l fructose g/l sucrose), H8(10 g/l sucrose), H2 (20 g/l glucose), and H11 (5 g/l fructose g/l sucrose) had significant effect on seed germination percentage. H11and H12 was the best medium for protocorm growth. H8P2(10 g/l sucrose g/l peptone), H2P2(20 g/l glucose g/l peptone) and H12P2(3.5g/l fructose g/l sucrose g/l peptone) were the best for seed germination respectively with 79/6%, 74/6% and 71/9% seed germination percentage. H10P2 (30 sucrose g/l g/l peptone) with 17/3mm growth, significantly was the best for protocorm growth. Therefore kind and concentration of carbohydrate and presence of organic nitrogen (peptone) influence asymbiotic seed germination percentage and protocorm growth and could improved both of them. This finding revealing that it is possible to improvement asymbiotic seed germination of this species orchid with combination of mono and disaccharides and organic nitrogen.