Evaluation of Toxicity and Anticancer Activity of Isolated Fraction from the Venom of Iranian Viper, Macrovipera lebetina on Lung Cancer Cell Line, TC-1
Message:
Abstract:
Background And Objectives
Cancer is a life threatening disease determined by uncontrolled differentiation and proliferation of cells. The cells escape apoptosis would be led to metastasis. Chemotherapy is the conventional method in treatment of cancer but the tumor often will be resistant to chemotherapy regimen leading to crucial side effects. Prevalence of lung cancer is 19.4% of all cancers. Many bio-toxins are useful source for biologically active compounds with anti-tumor activity. This study was aimed to finding an anti-cancer agent from the venom of Iranian snake viper, Macrovipera lebetina. The venom fractionated and anti-adhesion activity was examined. Candidate fraction selected and subjected to anion exchange chromatography to further fractionation. Subsequently, anti-adhesion and toxic activity determined on lung cancer line.
Mateials and
Methods
Viper venom prepared and separated by gel filtration chromatography using sephacryl S200.The concentration of fractions determined and anti-adhesion effect of the fractions evaluated on TC-1 cell line. Then, the active fraction was purified by anion exchange chromatography using MonoQ resin. Anti-adhesion activity and toxicity of resultant fraction determined on the cancer cells again. Toxicity of candidate fraction controlled on fibroblast cell line.
Result
Based on results six fractions were collected. Fraction 5 was active on cell lines and selected for anion exchange chromatography .Four anionic and two cationic fractions showed in the chromatogram and collected for anticancer activity. Fraction 3 has the most anti-adhesion activity. Totally anti-adhesion and toxicity on cancer cell line calculated as 40%. This fraction showed about 5% toxicity on fibroblasts.
Conclusion
An anti-adhesion fraction was detected in the venom of Iranian viper snake, Macrovipera lebetina. Toxicity of candidate fraction on cancer cells was less than anti-adhesion activity. Less toxicity was showed on fibroblast cells. Natural anti-cancer compounds derived from venomous animals can help fighting against cancer. It is the first report of an anti-adhesion fraction from an Iranian Viper with less toxicity on normal cells. Protein sequencing and in vivo study in mouse model of lung cancer would be suggested.
Language:
Persian
Published:
Iranian Journal of Infectious Diseases, Volume:22 Issue: 76, 2017
Page:
43
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