The Effects of 6 Weeks of Endurance Training and Consumption of Different Doses of Boldenone on Hematological Factors and Spleen Structure Changes in Male Wistar Rats

Regardless of how many periods and how long the androgenic-anabolic steroids have been used, they can cause side effects. The objective of this study was to investigate the effect a 6-week endurance training and using different doses of anabolic steroid boldenone on hematological factors and changes in spleen structure in male Wistar rats.
In this experimental study, 47 male Wistar rats aged 12 weeks, were randomly divided into 7 groups (control, sham, boldenone-1, boldenone-2, endurance training, endurance training溉嵺稧1, endurance training溉嵺稧2). Increasing endurance training program was performed at the speed of 10-30m/min (Vo2max, 75-80%) for 6 weeks and 5 days/week. The drug was injected deeply into the quadriceps and hamstring muscles once a week, on an appointed day. After anesthesia and dissection, the spleen was removed. Finally, the selected microscopic sections, were studied using a light microscope after staining with hematoxylin and eosin. Data were analyzed by dependent t-, one-way ANOVA, and post-hoc LSD tests at α In this study, boldenone supplementation at different doses led to weight gain, non-significant decrease in spleen weight (p=0.297), increase in white blood cells (p=0.041), and increase in hematocrit level (p=0.017). Also, there was a significant difference between the effect of exercise and boldenone consumption on the extent of damage to white pulp, red pulp, and the spleen sinusoidal space (p=0.001).
The findings of this study showed it is likely that short-term consumption of boldenone have negative effects on the spleen structure, followed by negative changes in hematological factors.