The Effect of Resveratrol on Expression of microRNA34a in U87MG Cancerous Cells Line

Glioblastoma multiform (GBM) is known as the most current and invasive kind of central nervous system tumors. In addition to existing therapy methods, using epigenetic factors specially investigation of microRNAs expression level has been known to predict prognosis, survival rate, and prevalence of a variety of cancers. In this study, the effect of resveratrol on expression level of miRNA34a and Snail gene in U87MG (ATCC® HTB-14™) cell line was investigated.
In this experimental research, U87MG (ATCC® HTB-14™) cell was used in culture medium content of 1% fetal bovine serum (FBS) with penicillin and streptomycin antibiotics. Resveratrol at different concentrations was used to determine 50% inhibitory effect on cell growth in cultured cells using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) method. Changes in microRNA and Snail gene expression were assessed using qualitative real-time polymerase chain reaction (qRT-PCR) method. ∆∆CT method was used to analyze gene expression data.
Findings: Assessing inhibitory effect of resveratrol at different concentration for 48 hours in culture medium showed that the concentration of 43.23 µg/ml was able to inhibit growth of 50% of treated cells. Gene expression analysis indicated that expression level of microRNA34a decreased while Snail gene expression significantly increased compared to control cells.
Resveratrol has ability to increase expression of microRNA34a but this increase did not resulted to decrease of Snail gene expression. So, it has not positive effect to control of metastasis pathway.