Assessment of Bifidobacterium bifidum ability to adsorption of Aflatoxin M1 in probiotic yoghurt

A method of eliminating aflatoxins including aflatoxin M1 is microbial detoxification. Reports indicate that some strains of lactic acid bacteria family through adsorption of aflatoxin in their cell wall can be effective in removing them. In this study the ability of Bifidobacterium bifidum in the adsorption of aflatoxin M1 in probiotic yoghurt was assessed. Thus, probiotic and starters (108 cfu/ml) were inoculated to restructured skimmed milk without aflatoxin M1. Then, the samples were spiked by aflatoxin M1 in different concentrations (0.1, 0.5 and 0.75 ppb). The concentration of aflatoxin residue in supernatant of probiotic yoghurt and control samples after different storage times (1, 7, 14 and 21 day) was measured by ELISA method and the results were confirmed by HPLC. The results showed that although, both probiotic and starters was able to adsorb aflatoxin M1, the ability of Bifidobacterium bifidum was higher. Also, the probiotic and starters were able to reduce the higher amount of toxin, when the toxin concentration was low. The results of survivability of Bifidobacteriunm bifidum showed that during storage time, the number of surviving cells decreased 4 logarithmic cycles.