Study of polymorphism in intron 4 and exon 5 of Ghrelin gene in Khouzestan native chickens using PCR-RFLP

This study was performed in order to identify polymorphism in fourth intron and fifth exon in Khuzestan province native chicken’s ghrelin gene using PCR-RFLP method. Therefore, blood sampling from 100 chickens of Native chickens in Khuzestan province breeding and Jihad livestock inputs randomly was performed. DNA extraction from blood samples was performed using salt method. The quantity and quality of DNA was determined using gel electrophoresis (1%) and Nanodrop respectively and then 228 bp fragment of fourth intron and fifth exon region was amplified using polymerase chain reaction (PCR). Eventually amplified samples were digested with HaeIII enzyme and digest parts of 173 and 55 bp were found. The results showed that in all the samples after enzymatic digestion was only a same band shape therefore, considering the same genotype samples examined in this study, mutation was not founded. Not found polymorphism may be due to closed nurture environment and the low number of samples.