Association Between Cytokine Gene Polymorphisms and Human Susceptibility to Brucellosis
Abstract:
Background
Allelic single nucleotide polymorphisms (SNPs) in cytokine-encoding genes can affect the degree of cytokine production, and may be related to the tendency to infectious illnesses as well as various clinical consequences.
Objectives
The aim of this work was to evaluate the possible role of SNPs in the regions of the IL-10 (-592), IL-15 (-367), IL-18 (-656), IL-12 (흟), IFN-γ (), TNF-α (-308), and TNF-β (�) genes in susceptibility or resistance to brucellosis and its crucial complications.
Methods
In a period of one year, 125 patients with acute brucellosis referring to 3 large public teaching hospitals were enrolled in this study. We studied the SNPs of IL-10, IL-15, IL-18, IL-12, IFN-γ, and TNF-α/β genes using the allele specific polymerase chain reaction (AS-PCR) with sequence-specific primers.
Results
Frequency of GG genotype in the TNF-α and TNF-β-encoding genes increased significantly by 52% and 31.2% in patient and control groups, respectively. For IFN-γ, TA genotype was found highly enhanced in patients (60%), while the frequency of AA and TT genotypes were higher in controls (23.2% and 26.4%, respectively). The AA and CC SNPs in IL-12 were dominant in both patient (78.4%) and control (14.4%) groups. In the patient group, the GG and TT genotypes had a higher frequency for genes encoding IL-15 (33.6%) and IL-18 (89.6%).
Conclusions
Based on the present study, some SNPs within the several cytokine genes, including TNF-α/β (-308/�), IFN-γ (), IL-15 (-367), IL-18 (-656), and IL-12 (흟) are related to the susceptibility or resistance to brucellosis. In order to approve the biological consequence of our results, additional investigations should be carried out in larger population groups.
Language:
English
Published:
Archives of Pediatric Infectious Diseases, Volume:5 Issue: 3, Jul 2017
Page:
11
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