The Synergistic Effect of Thymus vulgaris and Matricaria Chamomilla extracts on visceral pain in mice: Experimental study

Aims and Based on existing scientific information, Thymus vulgaris and Matricaria chamomilla are two kinds of herbs with some anti-inflammatory effects. There are some serious side effects such as psychological dependence or blood dyscrasia subsequent to using synthetic drugs in order to treat unpleasant feeling due to Visceral pain. In order to lessen the side effects, administration of herbal medicines could be helpful. The purpose of this study is evaluating the palliative effects of hydroalcholic extracts of Thymus vulgarisand Matricaria chamomilla and their Synergistic effects on visceral pain.
This experimental study was performed on 40 N-MRI male mice (36±4g) who were randomly divided into five groups: the Negative Control group treated with normal saline, Positive Control group treated with Flunixin meglumine(2 mg/kg) and the groups of Thymus vulgaris therapy(300mg/kg), Matricaria chamomilla therapy (300mg/kg) and the last one that received both Thymus and Matricaria (total 300mg/kg).These groups received drugs and hydroalcholic extracts with intraperitonial injection. The Mice were injected with acetic acid 0.6% (10ml/kg) to make visceral pain; 15 minutes after each intraperitonial administration, the palliative effects were recorded by counting the number of writhing in 30 minutes. The data was analyzed by SPSS statistical software and One-Way ANOVA (Tukey) test. The significant value was shown with (p Findings: Co-administration of Thymus vulgaris (150 mg/kg) and Matricaria chamomilla (150 mg/kg) showed a significant reduction of pain compared to negative control group(p0.001).
Co-administration of Thymus vulgaris and Matricaria chamomilla can be more effective than their single administration; however further clinical studies are necessary to find a suitable place for them among other visceral analgesics.