The influence of GL-9 peptide on A549 and human and animal blood cells

Context: Scientists are looking for new treatment options with fewer side effects using natural compounds such as peptides.
In this study, we used different concentrations of GL-9 peptide to evaluate its cytotoxic effect on human lung adenocarcinoma cells (A549) and normal human and bovine lymphocytes and erythrocytes cells was investigated.
12, 25 and 50 µg/ml of GL-9 peptide were used to examine its cytotoxic effect on A549cells after 24 h and 48 h via MTT assay. Furthermore, hemolysis and lympho-toxic assay were used to analyze GL-9 cytotoxic influence on human normal cells.
Our results showed the cytotoxic effect of GL-9 peptide on A549 cell line within 48 h. Moreover, our results showed no hemolytic and cytotoxic effect on human and bovine red and white blood cells.
Discussion and GL-9 peptide had cytotoxic effect on A549 cell line after 48h with no toxic activity on human and bovine peripheral blood mononuclear cells, therefore through further analysis and examination this peptide could be a replace for common cancer therapy drugs in the near future