shRNA-mediated downregulation of α-N-Acetylgalactosaminidase inhibits migration and invasion of cancer cell lines
Author(s):
Abstract:
Objective(s)
Extracellular matrix (ECM) is composed of many kinds of glycoproteins containing glycosaminoglycans (GAGs) moiety. The research was conducted based on the N-Acetylgalactosamine (GalNAc) degradation of ECM components by α-N-acetylgalactosaminidase (Nagalase) which facilitates migration and invasion of cancer cells. This study aims to investigate the effects of Naga-shRNA downregulation on migration and invasion of cancer cell lines.Materials And Methods
In this study, MCF-7 cell line (human mammary carcinoma cell line) and A2780 (human ovarian carcinoma cell line) were used. The level of normalized Naga expression and Nagalase protein were evaluated by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay/western blotting, respectively. Migration and invasion were determined using transwell assays, and statistical analysis was carried out by ANOVA test.Results
Response to transduction by shRNA compared to the control group, migrative and invasive properties of the transfected cells were significantly inhibited.Conclusion
These results indicate that Nagalase may have an important role in migration and invasion of cancer cells and can be considered as a candidate for further studies.Keywords:
Language:
English
Published:
Iranian Journal of Basic Medical Sciences, Volume:20 Issue: 9, Sep 2017
Pages:
1021 to 1028
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