Investigation of poly unsaturated fatty acid production from Mortierella alpine CBS 754.68 in hydrolyzation polysaccharide media

According to important of arachidonic acid in food chain, Mortierella alpine as good resource of arachidonic acid was considered by many scientists. In this research, alpha amylase of immobilized bacillus amyloliquefaciens was used for hydrolyzing of polysaccharide in order to production reduces sugar. These substrates that hydrolyzed and have high content of reduce sugar were fermented for fatty acid and biomass production by Mortierella alpine. The highest levels of reduce sugar were obtained in wheat sprouts (102.57), millet (57.50) and wheat (51.62) respectively. Starch and reduce sugar together in media has great effect on dry biomass. The highest and lowest content of biomass was obtained in wheat sprouts (3.7) and rice (1.72) respectively. The analysis of profile of fatty acid showed that the highest content of commercial fatty acid such as linoleic acid (41.873), oleic acid (30.61) and archidonic acid (27.54) were obtained in corn, rice and potato medium respectively.