Assessment of leukocyte changes, inflammatory markers and muscular damage in serum following the repair of traumatic muscular wounds in dogs

Occurrence of Inflammation process is a vital response induced by immune system for muscle repair. Following of muscular damage in traumatic wounds, immune system may play an important role in rehabilitation and repair of muscular and surrounding connective tissue. Aggregation of destructional proteins may initiate inflammatory responses which would be similar to acute phase reactions. Regulation of immune responses to muscular damage and inflammation is modulated by special messengers named cytokines including MCP-1. Increased activity of Keratin kinase and Lactate dehydrogenase values considered laboratory signals of muscular destructions. Additionally, leukocytes are participating in rehabilitation and repair of muscular damage. Therefore, the severity of immune reactions and inflammatory responses after muscular damage and also repair process would be assessed by leukocyte count, inflammatory markers and presenting cytokines in the blood. The aim of this study was to evaluate the immune and leukocyte responses in inflammation and repair process in macular traumatic wounds in dogs. To this point, 6 number of dogs with ulcer and traumatic wounds were selected, serum inflammatory and muscular markers and leukocyte changes were studied in the first referral and after wound repair. The results showed that neutrophilic leukocytosis and lymphocytosis, significant increase of cortisol level and MCP-1, CPK and LDH were observed in traumatic wounds. After tissue repair, CPK and LDH were returned to the basic value and leukocyte changes were found as monocytosis and slight leukocytosis and MCP1 value climbed markedly compared to the first referral. It would be concluded that released factors in the time of muscular damage and inflammation were not accelerated after performing appropriate treatment and repair and measurement of MCP-1 values would be helpful in determination of active repair process.