Genetic comparison of Garra rufa (Heckel, 1843) in Shahpour (Kazeron) and Berim (Gachsaran) rivers by using microsatellite markers

In this study, Population of Garra rufa was investigated using six microsatellite loci in Fars and Kohgiluyeh and Boyer-Ahmad province (Shahpour and Berim Rivers). In this study fulfilled on 60 Garra individuals (30 individuals for each river). The mean number of allele’s level at the population was 13.5 which are more than the reported values for freshwater fishes. The expected and observed heterozygosity were 0.658 and 0.865, respectively. Most cases deviated significantly from Hardy-Weinberg equilibrium (p≤0.01). It seems that there is a low differentiation between two populations of this species in the studied areas due to high level of gene flow (Nm=18.205) between two regions and low Fst (0.036). Also, analysis of molecular variance showed there is low genetic variation among populations and most of the observed variation is within the populations. a low differentiation between two populations of this species in the studied areas due to high level of gene flow (Nm=18.205) between two regions and low Fst (0.036). Also, analysis of molecular variance showed there is low genetic variation among populations and most of the observed variation is within the populations.