induction Effects of Colchicine and Chitosan on Rosmarinic Acid Production in Hairy Root Cultures of Zarrin-Giah (Dracocephalum kotschyi Boiss

Dracocephalum kotschyi Boiss belonging to Labiateae family is an herbaceous, pereninal and endemic plant known in Iran as Zarrin-Giah. Recent pharmacological studies demonstrated this plants with methoxylated flavonoids, have anti-cancer effects. Hairy roots were obtained from leaves explants, inoculated with 15834 strain of Agrobacterium rhizogenesis. In present study, The effects of biotic elicitors including, Colchicine with different cocentrations ( 0, 0.01, 0.03 and 0.05% (w/v)) and Chitosan (0, 50, 100 and 150 mg/l) for 48 hours on hairy root growth and Rosmarinic acid production were investigated. ANOVA results of Antioxidant activity, total phenol and total flavonoids showed significant difference among the various treatments (P