Optimization of recombinant laccase production by Yarrowia lipolytica in a medium containing glucose as carbon source with Taguchi method

Laccases (EC 1.10.3.2; benzenediol: oxygen oxidoreductase) are copper-containing oxidases that use molecular oxygen to oxidize various aromatic and non-aromatic compounds. Laccase is applied in delignification of lignocellulosic compounds for production of bioethanol, bioremediation of industrial wastewaters especially textile, food industries, and making biosensors.
The Taguchi experimental design method was used for optimization of laccase production in recombinant strain Yarrowia lipolytica YL4. A L-16 Taguchi orthogonal array was used to optimize the carbon and nitrogen sources along with vitamin in four levels.
The results showed that glucose, ammonium chloride, yeast extract and thiamine have significant effects on the production of laccase, respectively. The laccase activity reached to 1.52 U/mL after optimization of medium which is 7.6-fold higher than un-optimized medium.
Discussion and According to the analysis of results, the Taguchi experimental design method is a successful approach to increase laccase and recombinant proteins production in Y. lipolytica.