Sequencing and bioinformatics analysis of the exon 9 of Prolactin receptor gene in Khalkhali goat

Due to a crucial role in transferring of Prolactin hormone signals to milk protein gene promoter, Prolactin receptor (PRLR) gene has been detected as the most important candidate gene. The present study investigated the genetic variation of exon 9 of PRLR gene and bioinformatics analysis of this gene in farm animals. For bioinformatics analysis, 37 samples of different farm animal species genomic sequences were obtained. A mutation was detected in exon 9 of Khalkhali goat PRLR gene that causes to the substitution of A to G nucleotide and, led to change ATG codon to GTG codon with the substitution of Metioning amino acid with Valin. The results of analyzing of Exon 9 led to identify 28 polymorphic sites in the PRLR gene that causes 10 haplotypes with 0.8408 haplotype diversity in studied species. Nucleotide diversity and average nucleotide differences among species were 0.04439 and 10.655 respectively. Analysis of genetic diversity showed that ovine species have the more diversity compared with other species. Also, analysis of species distance and network analyses showed that ovine is most closed species to Capra than other species, and Khalkhali goat belongs to the same phylum as Capra Hircus.