Lipid Extraction from Dunalliela sp.by Ultrasonic Waves

In this research Dunalliela sp.M1 isolate was cultivated in open pound bio reactor. This isolate was harvested by electrocoagulation method from culture medium. The harvested biomass was dried and desalinated. Lipid extraction process was carried out by soxhelt method and the effect of extraction time (20- 40min), solvent type (Hexane or mixture of hexane-Isopropanol 3:2) and ultrasonic pre-treatment factors on amount of extracted lipid was evaluated. Test results showed that in soxhelt method the hexane and isopropanol solvents (3:2 ratio),during 40 minutes and by applying of ultrasonic pretreatment had the maximum lipid extraction (16.64%). According to statistics analysis increasing of extraction time had significant effect (p