Evaluation of Lethal Effect of Pergularia Tomentosa and Priploca aphylla on Trichomonas Vaginalis In Vitro

Trichomonas vaginalis protozoa is one of the causes of human vaginitis. The selective medicine for treatment is metronidazole that in addition to increasing drug resistance, it also has many side effects. Pergularia tomentosa is a dessert plant that has the anti-fungal and anti- Plasmodium effect and anti dermatophytic effect of Priploca aphylla is also more effective than common antifungal medicine such as griseofulvin. In this study, we hope to use of these plants to introduce a new medicine combination.
Pergularia tomentosa and Priploca aphylla were cleaned and dried after collecting, then each of the plants were extracted by using water, methanol, dicholoromethane and n-hexane solvents and with solvents mentioned concentrations 50, 100, 200, 400, 600, 800 µg/ml were prepared by using the normal saline and glycerin. 106 Trichomonas vaginalis trophozoite was added in 100 µl, then add 100 ml of the plants extract of Pergularia tomentosa and Priploca aphylla were added and incubated for 24 h at 37°C. Measurement of mortality rate of this protozoan were evaluated by using the MTT test. The effect of the extracts was performed on vero cells and Phytochemical evaluation was used by using the technique (HPTLC) to determine the composition of the extract. All tests wererepeated 3 times. SPSS 16 software were used for data analysis.
Trichomonas lethal effect of aqueous, methanol, dichloromethane and n-hexane extracts of Pergularia tomentosa in 800µg/ml concentration were % 64, % 72.4, %95.2 and % 95.4 respectively. anti Trichomonas effect of Priploca aphylla and with same concentration were 70.8%, 67%, 93.4% and 93.2% respectively. Cytotoxic effect of methanol extracts of Pergularia tomentosa and Priploca aphylla in 800µg/ml concentration against vero cells obtained %50.3 and %63 respectivley. Phytochemical screening of both plants extracts showed that both have the ingredients alkaloid, flavonoid, terpenoid, saponin, sterol, phenol, iridoid, tannin, phenylpropanoid, and anthocyanines.
results showed that the Pergularia tomentosa and Priploca aphylla, dichloromethane and n-hexane extracts had more potent effect against Trichomonas vaginalis than methanolic and water extracts in all concentrations. so, identification and evaluation of the mechanism of these ingredients against Trichomonas vaginalis is recommended.