Rapid molecular detection of Listeria monocytogenes in Iran industrial sauce

Aim and Listeria monocytogenes is known as a food-borne pathogen responsible for causing disease in humans. This bacterium that cause meningitis, abortion and food poisoning in humans. Industrial sauce as a matter of day-to-day offer food consumption among the general population have found. This food, suitable for food poisoning. Therefore contamination with Listeria monocytogenes is a threat to health, especially those with suppressed immune systems. The aim of this study is molecular detection of listeria monocytogenes in industrial sauce in Iran.
25 Industrial sauces were collected from Iran markets. DNA samples extracted by standard phenol-chloroform method . PCR test optimized and detection of limits (LOD) and specificity evaluated. All of the samples tested with optimized PCR test.
PCR test Optimized and 226 bp amplicon was observed in 2% agarose gel electrophoresis. LOD test was obtained 10 genome/reaction and in specificity test, amplicon was not observed with none of microorganism DNA was tested. After PCR testing on 25 sauce samples,4 were positive for Listeria monocytogenes.
PCR technique using specific primers very carefully and faster than traditional methods is for the identification of Listeria monocytogenes. The results show that the PCR technique is a good way to monitor microorganisms in food products such as sauces.