Role of HDM2 Gene in Radio-Sensitivity of Esophageal Cancer Cell Lines to Irradiation

Radio therapy plays an important role in controlling tumor growth in esophageal cancer patients.
Our study provided comprehensive information about radio-sensitivity of Hdm2 gene in esophageal cancer cells in cancer cells.
The expression of Hdm2 gene in radiosensitive and radio-resistant esophageal squamous cell carcinoma TE1, TE8 and TE11 cell lines is studied. RNA extracted from each esophageal cancer cell, then Quantitative reverse transcription PCR (RT-qPCR) assay were used for measuring HDM2 gene expression. Finally, radiosensitivity was determined for every three esophageal cancer cell lines.
It was found that Hdm2 gene significantly expressed in TE1, TE8 and TE11 cell lines by qRT-PCR analysis. Clonogenic assay showed that TE-11 was very sensitive to radiation relative to other cell lines.
Hdm2 protein binding to the P53 protein is a key regulator of cell cycle control and ýgenome protection Cell cycle control, programmed cell death and response cells to ýradition play important roles.ý