Effect of Different Levels of Guar Meal With β-mannanase Enzyme on Ileal Bacteria Population, Immune Response and Some Blood Parameters in Broiler Chickens

This experiment was conducted to investigate effects of different levels of Guar meal (0, 5, and 10%) and β-mannanase enzyme (0 and 0/05 percent) on microbial flora in Ileal, immune response and some blood parameters in broiler chickens during grower and finisher stages in 2×3 factorial arrangement with a randomized complete block design. Three hundred sixty Ross 308 male broiler chicks with 6 treatments and 4 replicates, 15 chicks in each experimental unit reared for 42 days. To evaluate the bacterial population in Ileum 42 day-old birds were slaughtered by cervical dislocation and after opening abdominal cavity a portion of Ileum were investigated for bacterial population. To investigate immune response one ml of sheep red blood cells was injected intramuscular to the birds at 35 day-old and blood samples were taken from wing vein at 41 day-old. In order to determine blood parameters from each treatment 4 birds were selected and blood samples for determining some blood parameters transferred to the laboratory. The results of this experiment showed that the lowest anaerobic, E.coli population observed in birds fed 10% Guar meal with enzyme supplementation (P