Evaluation of yield and secondary metabolites of mullein (Verbascum phlomoides L. var. Napfeny) in Shahrekord and Sepidan Regions

Mullein (Verbascum phlomoides L.) is a valuable medicinal plant belonging to the Scrophulariacea. In this study, the yield and active substances of flowers in Shahrekord and Sepidan regions were investigated. The experiment was based on a completely randomized design with four replications. Seeds were sown in autumn. Flowers were collected at full flowering stage for evaluation of yield and active substances. Total phenols, total anthocyanin, mucilage, total flavonoids and antioxidant activity were measured using Folin-ciocalteau, different pH, hot extraction, colorimetric aluminum, and DPPH methods, respectively. Shahrekord region had the highest flower yield (652.5 kg/h), total flavonoid (3.53 mg rutin/g dw), total anthocyanin (1.35 mg/ml) and antioxidant activity (117.42), showing significant difference with Sepidan region (P