Bacteriologic investigation of bovine mastitis in Isfahan livestocks and the effect of beehive Propolis and Pollen extracts on the isolated bacteria

Mastitis is inflammation which causes physical and chemical changes in milk and pathological changes in mammary gland. The aims of present study were identification of bovine mastitis causative agents in Isfahan and survey of bee Propolis and Pollen antibacterial effect on the isolated bacteria. Milk samples were aseptically obtained from 10 mastitic cattle. The isolated bacteria were detected based on macroscopic, microscopic and biochemical characteristics. Ethanol and acetone extracts were prepared from Propolis and Pollen by soaking and the antibacterial effects of them determined using well plate and microdilution methods. The isolated bacteria included Streptococcus agalactiae, Staphylococcus aureus, Corynebacterium sp., Listeria sp., Pseudomonas aeruginosa and Escherichia coli. Well plate method showed that Propolis and Polen extracts were effective on Gram-positive bacteria but had no effect on Gram-negative ones. The mean of results showed maximum effects of Pollen acetone extract on Streptococcus agalactiae (concentration of 12.5 mg/ml) and Pollen ethanol extract on Corynebacterium (concentration of 12.5 mg/ml). Acetone extract of Propolis showed maximum effect on Staphylococcus aureus (concentration of 25 mg/ml) and Listeria sp. (concentration of 12.5 mg/ml). The results were confirmed by minimum bactericidal concentration analysis. Extracts of propolis and pollen showed powerful effects on Gram-positive isolates and this effect was more detected by acetone extracts.