In Vitro Antileishmanial Activity of Falcaria vulgaris Fractions on Leishmania major
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background
In recent years, many reports have been published regarding the increasing frequency of drug resistance in Leishmania species to antimonial compounds. Accordingly, many researchers have tried to develop new medicines for the treatment of cutaneous leishmaniasis.
Objectives
The aim of this study was to evaluate the in vitro antileishmanial activity of aqueous, ethyl acetate, and butanol fractions of Falcaria vulgaris against Leishmania major promastigotes.
Methods
The total extract was obtained using the percolation method. Subsequently, chlorophyll and oil were eliminated from the total extract, using medium pressure liquid chromatography (MPLC), followed by liquid-liquid extraction (LLE) for fractionation of the total extract. The antileishmanial activity of each fraction was examined at 6 concentrations (40, 80, 160, 320, 640, and 1280 µg/mL) and 4 intervals (6, 24, 48, and 72 hours).
Results
The results of repeated measures ANOVA showed P values of 0.16, 0.22, and 0.04 for the aqueous, ethyl acetate, and butanol fractions, respectively. Furthermore, one-way ANOVA test revealed a significant difference between these fractions at 48 and 72 hours (P = 0.04 for both). In addition, Tukey’s HSD test showed a significant difference between the butanol fraction and other fractions at 48 and 72 hours (P
Conclusions
As Falcaria vulgaris showed acceptable antileishmanial activity with no side effects in this study, it can be used as an effective complementary or alternative treatment for skin lesions of cutaneous leishmaniasis. However, further in vivo studies are required in the future.
Language:
English
Published:
Jundishapur Journal of Natural Pharmaceutical Products, Volume:12 Issue: 3, Jun 2017
Page:
10
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