Early detection of Toxoplasma gondii by real-time polymerase chain reaction methods in patients with recurrent spontaneous abortions

One of the causes of recurrent spontaneous abortions (RSA) is an infection by the toxoplasmosis Protozoa. In comparison, we present detailed results using real-time polymerase chain reaction (PCR) methods of detection. In this study, it was tried to detect Toxoplasma gondii (T. gondii) by real-time PCR methods in patients with RSA.
Amniotic fluid sampling was performed in the 16-20th weeks of gestation in 50 pregnant women with a history of RSA. The extracted deoxyribonucleic acid (DNA) samples were analyzed using quantitative real-time PCR.
In all the cases, the detection of T. gondii was negative in the peripheral blood, and amniotic fluid samples by using the molecular methods (real-time PCR). Using the serological detection methods, 6% of patients were diagnosed as positive for the immunoglobulin M (IgM) antibody. In addition, the IgG antibody was positive in 46% of the patients.
It can be concluded that the serological methods lack specificity.