The effect of different concentrations of benzylaminopurine and naphthalene acetic acid on direct regeneration of explants of four Persian shallot (Allium hirtifolium) ecotypes at in vitro condition

In the present study, the effects of different concentrations of benzylamino purine (0, 1, 1.5 and 2 mg l-1) and naphthalene acetic acid (0, 0.25, 0.5 and 1 mg l1-) on direct and indirect bulblet rejected set by user of basal plate explants of four ecotypes of Psersian shallot (Lorestan, Zanjan, Sanandaj and Arak) on MS medium were investigated. The results showed that different hormonal treatments and ecotypes affected the time and percent of bulblet regeneration as well as the number, length and weight of regenerated bulblets. Moreover, direct and indirect regeneration of bulblet were different among the treatments. Application of BAP and NAA accelerated the bulblet regeneration of Persian shallot explants and the highest bulblet regeneration (82.26%) and maximum bulbllets number (14.13) were obtained by 1.5 mg g-1 BAP with 0.5 mg l-1 NAA. The explants of Arak ecotype showed the highest percent of bulblet regeneration (64.61%) and bulblets number (10.33) per each explant. The callus induction was only observed in 2 mg l-1 BAP mg l-1 NAA (75.05%) and 1 mg l-1 BAP.5 mg l-1 NAA (23.67%) treatments. The rooting of bulblet in all the studied ecotypes was obtained in hormone-free medium in the shortest time (25 after culture) in comparison with other treatments that was also accompanied with the highest percent of rooting (79.917%). According to the results the culture of Arak ecotype bulbs in medium with 1.5 mg g-1 BAP and 0.5 mg l-1 NAA is advisable for invitro bulblets production of Persian shallot.