The effect of Platelets desensitization on the levels of residual platelets reactivity in the diabetes mellitus type 2 patients

Background and Objectives Patients with diabetes mellitus type 2 have accelerated atherosclerosis. Hyperactive platelets and platelets micro particles play an important role in this prothrombotic state. Desensitization is a physiological process which plays an important role in turning off receptor mediated signal transduction pathways. In this study, we described and compared this process in diabetes mellitus type 2 patients and healthy individuals.
Materials and Methods In this fundamental-applied study, 40 diabetes mellitus type 2 patients and 35 healthy individuals participated in the study. After centrifuging at 200 g, PRP was prepared. Desensitization was induced with mild agonist stimulation and low dose of ADP (1 μM). Expression of platelets CD62P and CD41+ Annexin-v micro particles were evaluated by Flow cytometry. Data were analyzed by Anova and t-test.
Results In comparison with healthy individuals, the diabetic group showed a significant increase in expression of CD62P in baseline. Desensitized platelets had less expression of CD62Pb but these platelets produced more micro particles in this state. Diabetic group showed a significant increase in production of platelet micro particles in all of states with ADP treatment. (p ≤ 0.004)
Conclusions The flow cytometric data show that platelets in patients with diabetes mellitus type 2 patients are hyperactive that it associated to metabolic conditions. Induction of desensitization state helps platelets to reduce platelets activation and platelets sensitivity to ADP in diabetic environment. Also the production of platelets microparticles is high in these patients and desensitized platelets are more susceptible to microparticle shedding.