Application of Molecularly Imprinted Solid-Phase Extraction Coupled with HPLC for Preconcentration and Trace Determination of Celecoxib in the Human Urine Samples: Optimization by Central Composite Design (CCD)
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
A Molecularly Imprinted Solid-Phase Extraction (MISPE) coupled with High-Performance Liquid Chromatography with UV detection (HPLC/UV) described for preconcentration and trace determination of celecoxib in human urine samples. The synthesis of the Molecularly Imprinted Polymer (MIP) was performed by a non-covalent approach using methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker monomer, chloroform as the solvent, 2,2′-azobisisobutyronitrile (AIBN) as the initiator and celecoxib template as the target drug. Non-molecularly Imprinted Polymer (NIP) was also synthesized for comparison. Scanning Electron Microscopy (SEM) and FT-IR spectroscopy were used for characterizing the synthesized polymers. Also, a Central Composite Design (CCD) under Response Surface Methodology (RSM) and chemometrics applied to investigate and optimize the MISPE procedure parameters such as pH, loading and eluent solvent flow-rate, eluent solvent volume and sorbent mass, that probably influence the extraction process, to achieve the highest celecoxib extraction efficiency. Batch rebinding capacity of celecoxib was determined from the derived Langmuir isotherm and was found to be 15.06 mg/g. The Limit of Detection (LOD) and Limit of Quantification (LOQ) of this proposed method were 8 ng/mL and 26.7 ng/mL, respectively. The recoveries were 92.60-102% and the Relative Standard Deviation (RSD %) was lower than 4.65% in spiked urine samples. Accordingly, the proposed MISPE-HPLC/UV method can be used for the separation of trace amounts of celecoxib in human urine samples.
Language:
Persian
Published:
Iranian Journal of Chemistry & Chemical Engineering, Volume:36 Issue: 2, 2017
Pages:
87 to 104
magiran.com/p1787935  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!