Changes in Interleukin-2 and Interleukin-12 Cytokines in Spleen Cells of Balb-C Mice After Using an Acute Endurance Exercise as An Adjuvant for Herpes Simplex Virus 1 Vaccine

For vaccine efficacy always using adjuvant has been considered by immunologists. Exercise can induce immune changes, and therefore, has been identified as an adjuvant for immune responses. The purpose of this study was to assess adjuvant effects of an acute endurance exercise on interleukin-2 and interleukin-12 cytokines of spleen cells in herpes simplex virus 1 vaccine model. 24 BALB/c mice, 4 to 5 weeks old and with an average weight of 17.6 g, were divided into 3 groups: control, vaccine and vaccine plus an acute endurance exercise. Two weeks after three booster shots of vaccine, interleukin-2 and interleukin-12 levels was determined in spleen cell culture with ELISA method. One-ways ANOVA statistical test were used for data analysis. The result of this study indicated that there are significant differences between groups in interleukin-2 (P=0.008). Tukey post hoc test results showed significant differences between control and vaccine plus an acute endurance exercise groups (P=0.006). Changes in interleukin-12 cytokine production in vaccine and endurance exercise group were not significant. Result of current study showed that interleukin-2 levels from spleen cells increased following using an acute endurance exercise as an adjuvant. It seems that using an acute endurance exercise probably increased efficacy of immune system in direction to T helper 1 cells against virus infections.