Compare production of taxol in natural tissues of Taxus baccata L. and Taxus berevifolia Nutt. with in vitro condition
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Taxol has been introduced as an efficient natural compound with low toxicity in the control and treatment of a wide range of cancers, including breast, uterine, ovarian and other types that its production primary source is yew trees. Indeed, the limited number of yew trees and the impossibility of direct procurement of taxol from organs natural due to the legal ban on felling of these trees have been led to use in vitro cultures as a suitable alternative for the preparation of taxol. Nowadays, plant cell, tissues and organs cultures can provide the massive and rapid propagation of many medicinal plants. The present study focused on investigating, determining and comparing the amount of produced taxol with natural tissues of Taxus baccata L. and Taxus berevifolia Nutt., in vitro. In order to induce callus, stem and leaf explants were used in WPM medium enriched with 2,4-D and Kinetin hormones. The available Taxol was measured using HPLC techniques. After the extraction process, taxol was measured and compared in normal tissues including the skin, stems and leaves. The result showed that the amount of taxol in normal tissues of both species had a similar trend so that in the leaves was more than in the stem and then in the skin, but its amounts was different in both mentioned species. It was further observed that the amount of taxol was higher in T. baccata. Moreover, the amount of taxol in calluses of T. brevifolia and T. baccata were higher than normal tissues.
Language:
Persian
Published:
Journal of Plant Research, Volume:30 Issue: 1, 2017
Pages:
159 to 172
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