The inhibitory effect of Lactobacillus rhamnosus LGG 23527on growth and enterotoxin A gene expression of S. aureus ATCC 29213
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Increase shelf life, food safety and human health improvement by using the natural microflora are the dominant issues in public health. In this research, the effect of Lactobacillus rhamnosus (LGG) isolate with intestinal origin was studied on growth and enterotoxin production by Staphylococcus aureus and then, Staphylococcus aureus enterotoxin A gene expression was evaluated in a laboratory media. Therefore, this strain (1*107 cfu / ml) and Staphylococcus aureus (1*105 cfu / ml) were inoculated in TSB and incubated in two temperatures at 25 and 35°C, at the time of zero, 24, 48 and 72 hours, the mixed cultures were counted in specific media. Staphylococcus aureus without lactobacillus was incubated as control and then enterotoxin production evaluated by using ELISA kit and the gene expression of the SEA of S. aureus in mixed and control cultures was also evaluated by RT Real Time PCR technique. The lactobacillus isolates decreased Staphylococcus aureus growth compared to control samples 3 log at 25°C and 2 log at 35°C. The enterotoxin production of A, C and E at 25° and enterotoxin type E and A at 35°C was inhibited by Lactobacillus rhamnosus. Comparison of gene expression SEA between treated and control cultures showed the gene expression of the SEA was significantly suppressed 6.82 fold in S. aureus co cultured with studied lactobacillus isolate at 25°C while it was not significant to 35 °C (P
Language:
Persian
Published:
Iranian Veterinary Journal, Volume:13 Issue: 57, 2018
Pages:
9 to 18
magiran.com/p1793071  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!